基础研究:灯盏细辛对NMDA所致大鼠视网膜神经元损伤的保护作用_英文
2007-08-08
石晶明１，蒋幼芹１，刘旭阳２
Abstract
·AIM: To investigate whether Erigeron Breviscapus
(vant ) Hand-Mazz (EBHM) EBHMhas neuro protective ef -
fect against N-methyl-D-aspartate (NMDA)-induced neu -
ron death in retinal gan glion cell layer (RGCL).
·METHODS: 60 healthy SD rats were randomly divided
into four groups . 6 animals were normal control group
(group A). The others were divided as group B (EBHM
group ), group C (normal saline+NMDA group ) and group
D (EBHM+ NMDA group ). Each group had 18 rats .
10nmol NMDA was intravitreally injected to induce par -
tial damage of the neurons in RGCLin the right eyes of
Groups C and D. Same volume PBS was intravitreally injected
into the left eyes as self-control . Groups B and D
were pre-treated intraperitoneally with 6g/L EBHMsolution
at a dose of 150mg/kg body weight/day seven days
be fore and after NMDAtreat ment . Group C were admin -
istrated intraperitoneally with 9g/L normal saline at the
same time of EBHMinjection . Rats were sacrificed at 4,
7,14d after NMDA treat ment . Flat whole retinas were
stained with 5g/L cresyl violet and neuron count ing in
RGCL from both eyes were observed . Each sub group
had 6 rats .
·RESULTS: There was no significant difference of neu -
ron counting in RGCLbetween the right eye and the left
eye in group A (P =0 .200 ). There was no significant differ
ence between normal control group and EBHMgroup
either in the right eyes or in the left eyes at 4, 7 and 14
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